rabbit polyclonal antibodies against fn1  (Proteintech)

Journal: Cerebral Circulation - Cognition and Behavior

Article Title: A novel gene edited rat model of cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy (CARASIL)

doi: 10.1016/j.cccb.2025.100401

Figure Lengend Snippet: Increased FN1 and LTBP-4 in cerebral arterioles of CRHTRA1 rats. Measurement of FN1 expression in pial arteries was conducted using automated threshold intensity quantification. Vessels were captured from regions of interest identified during vessel thickness quantification. Compared to WT rats (A), CRHTRA1 rats showed significantly increased fluorescent intensity of FN1 in pial arteries (B, C). Similarly, compared to WT rats (D), CRHTRA1 rats showed significantly increased fluorescent intensity of LTBP-4 in pial arteries (E, F). Scale bars = 100 µm. Data shown as mean ± SD of vessels imaged from n = 6 CRHTRA1 rats and n = 6 WT rats. * p < 0.05; *** p < 0.001.

Article Snippet: Extracellular matrix (ECM) protein expression was examined using either rabbit polyclonal anti-fibronectin-1 (FN1) (ProteinTech, 15613–1-AP, 1:200) or mouse monoclonal anti-LTBP-4 (R&D Systems, Minneapolis, MN, AF2885, 1:100).

Techniques: Expressing

Journal: iScience

Article Title: Mechanism of hsa_circ_0069443 promoting early pregnancy loss through ALKBH5/FN1 axis in trophoblast cells

doi: 10.1016/j.isci.2024.111608

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-FN1 , Proteintech , Cat#:15613-1-AP; RRID: AB_2105691.

Techniques: Recombinant, Magnetic Beads, cDNA Synthesis, SYBR Green Assay, Membrane, Blocking Assay, Red Blood Cell Lysis, Selection, Methylation, Extraction, Cell Counting, CCK-8 Assay, Software

Journal: Journal of Molecular Cell Biology

Article Title: CCT6A alleviates pulmonary fibrosis by inhibiting HIF-1α-mediated lactate production

doi: 10.1093/jmcb/mjae021

Figure Lengend Snippet: Activation of lactate signal promotes pulmonary fibrosis in mice. ( A ) Timeline of GPR81 agonist-treated mouse lung fibrosis model. ( B ) Quantification of hydroxyproline content in mouse right inferior lobe ( n = 4 mice per group). ( C ) Quantification of total cells in BALF ( n = 3). ( D ) Protein concentration in BALF ( n = 3). ( E ) Representative images of H&E and Masson's Trichrome staining in mouse lung sections ( n = 3). Scale bar, 50 μm. The Ashcroft score was determined to indicate the severity of fibrosis. ( F ) Western blotting and quantification of fibrosis marker expression in whole lung lysates ( n = 3). ( G and H ) qPCR analysis of α-SMA, Col1a1, and Fn1 mRNA expression levels and immunofluorescence staining of lipid droplet in mouse lung tissue samples ( n = 4). Scale bar, 50 μm. * P < 0.05, ** P <0.01, *** P < 0.001, **** P <0.0001.

Article Snippet: FN1 , Polyclonal rabbit , Proteintech, 15613-1-AP , 1:2000.

Techniques: Activation Assay, Protein Concentration, Staining, Western Blot, Marker, Expressing, Immunofluorescence

Journal: Journal of Molecular Cell Biology

Article Title: CCT6A alleviates pulmonary fibrosis by inhibiting HIF-1α-mediated lactate production

doi: 10.1093/jmcb/mjae021

Figure Lengend Snippet: Cct6a restores BLM-induced lung fibrosis in mice. ( A ) Timeline of AAV-treated mouse lung fibrosis model. ( B ) Hydroxyproline in the right inferior lobe (Vehicle Sal n = 7, Cct6a Sal n = 8, Vehicle BLM n = 8, Cct6a BLM n = 7). ( C ) Total cell counts in BALF ( n = 7 mice per group). ( D ) Protein concentration in BALF (Vehicle Sal n = 7, Cct6a Sal n = 7, Vehicle BLM n = 8, Cct6a BLM n = 9). ( E ) Representative images of micro CT scans of mouse lung density ( n = 3). ( F ) Representative images of H&E and Masson's Trichrome staining in mouse lung sections ( n = 3). Boxed regions in the right superior panel are panoramic images of the lung tissue sections. Scale bar, 50 μm. The Ashcroft score was determined to indicate the severity of fibrosis. ( G ) Lactate level in mouse serum (Vehicle Sal n = 8, Cct6a Sal n = 10, Vehicle BLM n = 9, Cct6a BLM n = 10). ( H ) Western blotting and quantification of fibrosis marker expression in whole lung lysates ( n = 3). ( I ) qPCR analysis of Col1a1, Col3a1, Fn1, and Ctgf mRNA expression levels in the lung homogenate ( n = 6). ( J ) Immunoblot analysis of Vhl, Hif-1α, and Ldha protein levels in whole lung lysates ( n = 3). ( K ) Immunofluorescence staining of lipid droplet in mouse lung sections ( n = 3). Scale bar, 50 μm. * P < 0.05, ** P <0.01, *** P < 0.001, **** P <0.0001.

Article Snippet: FN1 , Polyclonal rabbit , Proteintech, 15613-1-AP , 1:2000.

Techniques: Protein Concentration, Micro-CT, Staining, Western Blot, Marker, Expressing, Immunofluorescence

Journal: Journal of Molecular Cell Biology

Article Title: CCT6A alleviates pulmonary fibrosis by inhibiting HIF-1α-mediated lactate production

doi: 10.1093/jmcb/mjae021

Figure Lengend Snippet: Primers and oligonucleotides.

Article Snippet: FN1 , Polyclonal rabbit , Proteintech, 15613-1-AP , 1:2000.

Techniques:

Journal: Journal of Molecular Cell Biology

Article Title: CCT6A alleviates pulmonary fibrosis by inhibiting HIF-1α-mediated lactate production

doi: 10.1093/jmcb/mjae021

Figure Lengend Snippet: List of antibodies used in this study.

Article Snippet: FN1 , Polyclonal rabbit , Proteintech, 15613-1-AP , 1:2000.

Techniques: Ubiquitin Proteomics

Journal: eLife

Article Title: CHD-associated enhancers shape human cardiomyocyte lineage commitment

doi: 10.7554/eLife.86206

Figure Lengend Snippet:

Article Snippet: Antibody , Anti-human-FN1 (Rabbit polyclonal) , Thermo Fisher , Cat#PA5-29578 , WB(1:1000).

Techniques: Hybridization, Knock-Out, Sequencing, Recombinant, Plasmid Preparation, Software, Electrophoresis, Quantitation Assay, Imaging, Microscopy

Journal: Journal of Orthopaedic Translation

Article Title: Pharmacological network analysis of the functions and mechanism of kaempferol from Du Zhong in intervertebral disc degeneration (IDD)

doi: 10.1016/j.jot.2023.01.002

Figure Lengend Snippet: The compound-target-signaling networks generated using Cytoscape_v3.8.0. The active compounds cyclopamine, quercetin, kaempferol and mairin could target 12 IDD disease genes including HMGB1, PTEN, IGFBP6, FGFR3, COL3A1, MMP2, SMAD2, HSPG2, GPC1, FN1, CUL4B, CSGALNACT1 (Among 13 target genes, INTS8 did not have pathway enrichment). The compound-targeted disease genes were enriched in 23 pathways.

Article Snippet: The following primary antibodies were used in this study: p16 (CSB-PA003618, CUSABIO, Wuhan, China), p21 (ab109520, Abcam, Cambridge, UK), Rb (ab224426, Abcam), hTERT (DF7129, Affinity Biotech), Nrf2 (CSB-RA225569A0HU,Cusabio), HO-1 (10701-1-AP, Proteintech), NQO-1 (11451-1-AP, Proteintech), SOD1 (10269-1-AP, Proteintech), SOD2 (ab227091, Abcam), Cleaved-caspase 3 (ab2302, Abcam), Caspase 3 (ab13847, Abcam), Bax (50599-2-Ig, Proteintech), Bcl-2 (12789-1-AP, Proteintech), aggrecan (13880-1-AP, Proteintech), collagen II (CSB-PA005739ESR2HU, Cusabio), SOX9 (ab185966, Abcam), FN1 (AF5335, Affinity Biotech), MMP2 (CSB-PA003258, Cusabio), MMP3 (17873-1-AP, Proteintech), MMP13 (CSB-PA07029A0Rb, Cusabio), ADAMTS-4 (DF6986, Affinity Biotech), ADAMTS-5 (DF13268, Affinity Biotech), p-p38 (AF4001, Affinity Biotech), p38 (14064-1-AP, Proteintech), p-JNK (AF3318, Affinity Biotech), JNK (AF6319, Affinity Biotech), p-ERK1/2 (sc-81492, Santa Cruz, Dallas, DX, USA), ERK1/2 (16443-1-AP, Proteintech).

Techniques: Generated

Journal: Journal of Orthopaedic Translation

Article Title: Pharmacological network analysis of the functions and mechanism of kaempferol from Du Zhong in intervertebral disc degeneration (IDD)

doi: 10.1016/j.jot.2023.01.002

Figure Lengend Snippet: Compound-target-signaling networks.

Article Snippet: The following primary antibodies were used in this study: p16 (CSB-PA003618, CUSABIO, Wuhan, China), p21 (ab109520, Abcam, Cambridge, UK), Rb (ab224426, Abcam), hTERT (DF7129, Affinity Biotech), Nrf2 (CSB-RA225569A0HU,Cusabio), HO-1 (10701-1-AP, Proteintech), NQO-1 (11451-1-AP, Proteintech), SOD1 (10269-1-AP, Proteintech), SOD2 (ab227091, Abcam), Cleaved-caspase 3 (ab2302, Abcam), Caspase 3 (ab13847, Abcam), Bax (50599-2-Ig, Proteintech), Bcl-2 (12789-1-AP, Proteintech), aggrecan (13880-1-AP, Proteintech), collagen II (CSB-PA005739ESR2HU, Cusabio), SOX9 (ab185966, Abcam), FN1 (AF5335, Affinity Biotech), MMP2 (CSB-PA003258, Cusabio), MMP3 (17873-1-AP, Proteintech), MMP13 (CSB-PA07029A0Rb, Cusabio), ADAMTS-4 (DF6986, Affinity Biotech), ADAMTS-5 (DF13268, Affinity Biotech), p-p38 (AF4001, Affinity Biotech), p38 (14064-1-AP, Proteintech), p-JNK (AF3318, Affinity Biotech), JNK (AF6319, Affinity Biotech), p-ERK1/2 (sc-81492, Santa Cruz, Dallas, DX, USA), ERK1/2 (16443-1-AP, Proteintech).

Techniques: Activity Assay, Migration

Journal: Journal of Orthopaedic Translation

Article Title: Pharmacological network analysis of the functions and mechanism of kaempferol from Du Zhong in intervertebral disc degeneration (IDD)

doi: 10.1016/j.jot.2023.01.002

Figure Lengend Snippet: Effects of Kaempferol IL-1β-induced ECM deposition NPCs were exposed to IL-1β stimulation (10 ​ng/ml, 24 ​h), treated with 10 ​μM kaempferol, and examined for the mRNA and protein levels of aggrecan, collagen II, SOX9, FN1 (A–B), MMP3, MMP13, ADAMTS-4, and ADAMTS-5 (C–D) using qRT-PCR and Immunoblotting, respectively. . One-way ANOVA followed by Tukey's post hoc test. N ​= ​3, ∗∗p ​< ​0.01, compared to PBS group; #p ​< ​0.05, ##p ​< ​0.01, compared with IL-1β group.

Article Snippet: The following primary antibodies were used in this study: p16 (CSB-PA003618, CUSABIO, Wuhan, China), p21 (ab109520, Abcam, Cambridge, UK), Rb (ab224426, Abcam), hTERT (DF7129, Affinity Biotech), Nrf2 (CSB-RA225569A0HU,Cusabio), HO-1 (10701-1-AP, Proteintech), NQO-1 (11451-1-AP, Proteintech), SOD1 (10269-1-AP, Proteintech), SOD2 (ab227091, Abcam), Cleaved-caspase 3 (ab2302, Abcam), Caspase 3 (ab13847, Abcam), Bax (50599-2-Ig, Proteintech), Bcl-2 (12789-1-AP, Proteintech), aggrecan (13880-1-AP, Proteintech), collagen II (CSB-PA005739ESR2HU, Cusabio), SOX9 (ab185966, Abcam), FN1 (AF5335, Affinity Biotech), MMP2 (CSB-PA003258, Cusabio), MMP3 (17873-1-AP, Proteintech), MMP13 (CSB-PA07029A0Rb, Cusabio), ADAMTS-4 (DF6986, Affinity Biotech), ADAMTS-5 (DF13268, Affinity Biotech), p-p38 (AF4001, Affinity Biotech), p38 (14064-1-AP, Proteintech), p-JNK (AF3318, Affinity Biotech), JNK (AF6319, Affinity Biotech), p-ERK1/2 (sc-81492, Santa Cruz, Dallas, DX, USA), ERK1/2 (16443-1-AP, Proteintech).

Techniques: Quantitative RT-PCR, Western Blot

Journal: Journal of Orthopaedic Translation

Article Title: Pharmacological network analysis of the functions and mechanism of kaempferol from Du Zhong in intervertebral disc degeneration (IDD)

doi: 10.1016/j.jot.2023.01.002

Figure Lengend Snippet: Table S1 The primers for qRT-PCR

Article Snippet: The following primary antibodies were used in this study: p16 (CSB-PA003618, CUSABIO, Wuhan, China), p21 (ab109520, Abcam, Cambridge, UK), Rb (ab224426, Abcam), hTERT (DF7129, Affinity Biotech), Nrf2 (CSB-RA225569A0HU,Cusabio), HO-1 (10701-1-AP, Proteintech), NQO-1 (11451-1-AP, Proteintech), SOD1 (10269-1-AP, Proteintech), SOD2 (ab227091, Abcam), Cleaved-caspase 3 (ab2302, Abcam), Caspase 3 (ab13847, Abcam), Bax (50599-2-Ig, Proteintech), Bcl-2 (12789-1-AP, Proteintech), aggrecan (13880-1-AP, Proteintech), collagen II (CSB-PA005739ESR2HU, Cusabio), SOX9 (ab185966, Abcam), FN1 (AF5335, Affinity Biotech), MMP2 (CSB-PA003258, Cusabio), MMP3 (17873-1-AP, Proteintech), MMP13 (CSB-PA07029A0Rb, Cusabio), ADAMTS-4 (DF6986, Affinity Biotech), ADAMTS-5 (DF13268, Affinity Biotech), p-p38 (AF4001, Affinity Biotech), p38 (14064-1-AP, Proteintech), p-JNK (AF3318, Affinity Biotech), JNK (AF6319, Affinity Biotech), p-ERK1/2 (sc-81492, Santa Cruz, Dallas, DX, USA), ERK1/2 (16443-1-AP, Proteintech).

Techniques: